The Pseudomonas aeruginosa Initiation Factor IF-2 Is Responsible for Formylation-independent Protein Initiation in P. aeruginosa

The interaction of radioactive initiation factor IF-2 with ribosomes during initiation of protein synthesis.

The interaction of initiation factor IF-Z with ribosomes during initiation of protein synthesis was studied in vifro with the radioactive factor, [32P]phosphoryl initiation factor IF-Z. The binding and release of the factor were analyzed by sucrose density gradient centrifugation. The results show that: 1. Initiation factor IF-2 is part of the complete 30 S initiation complex formed in the pres...

The Pseudomonas aeruginosa flagellar cap protein, FliD, is responsible for mucin adhesion.

Mucin-specific adhesion of Pseudomonas aeruginosa plays an important role in the initial colonization of this organism in the airways of cystic fibrosis patients. We report here that the flagellar cap protein, FliD, participates in this adhesion process. A polar chromosomal insertional mutation in the P. aeruginosa fliD gene made this organism nonadhesive to mucin in an in vitro mucin adhesion ...

Anaerobic regulation of transcription initiation in the arcDABC operon of Pseudomonas aeruginosa.

The arcDABC operon of Pseudomonas aeruginosa encodes the enzymes of the arginine deiminase pathway, which is inducible under conditions of oxygen limitation and serves to generate ATP from arginine. The 5' end of arc mRNA extracted from anaerobically grown cells was determined by S1 and primer extension mapping. The transcription initiation site was located upstream of the arcD gene and 41.5 bp...

Encapsulated Pseudomonas Aeruginosa (pseudomonas Aeruginosa Mucosus) Strains.

Construction of New Genetic Tools as Alternatives for Protein Overexpression in Escherichia coli and Pseudomonas aeruginosa

Background: Pseudomonas protein expression in E. coli is known to be a setback due to signifi cant genetic variation and absence of several genetic elements in E. coli for regulation and activation of Pseudomonas proteins. Modifi cations in promoter/repressor system and shuttle plasmid maintenance have made the expression of stable and active Pseudomonas protein possible in bot...